ABSTRACT
Objective To investigate the correlation between nuclear factor-associated factor 2 gene polymorphism and COPD and the effect of tiotropium bromide combined with budesonide/formoterol inhalation. Methods Seventy-nine patients with chronic obstructive pulmonary disease and 54 healthy subjects were enrolled to determine the NRF2-617C/A(rs6721961)polymorphism by PCR-RFLP and to analyze the relationship between different genotypes and COPD ,and then to observe the therapeutic effect of different genotypes. Results The frequency of A allele in NRF2-617C/A(rs6721961)was a risk factor for COPD(OR=2.312,95%CI:1.517 to 6.824,P<0.05). The total effective rate of AA was lower than that of CC and CA(χ2=8.65,7.24,P<0.05). After treatment,FEV1% pred,FEV1/FVC in the AA type were significantly lower in the CC type(t=6.48,5.62, P<0.05),while IL-8,TNF-αand hs-CRP were significantly higher than those in the CC type(t=6.07,9.21, 4.79,P<0.05). Conclusion NRF2-617C/A(rs6721961)gene polymorphism has a certain correlation with COPD,on which it has a significant effect to tiotropium combined with budesonide treatment and has a moderating effect on inflammatory response and pulmonary function in COPD patients.